منابع مشابه
Secretion of thermostable DNA polymerase using a novel baculovirus vector.
Baculovirus-mediated expression has become a powerful tool for the high yield production of functionally active recombinant proteins. In order to further enhance the utility of this expression system, we constructed versatile transfer vectors that facilitate the secretion of recombinant proteins from host insect cells by inserting functional secretory leader sequences down-stream of the polyhed...
متن کاملCharacterization of the Human Dna Polymerase Δ Catalyticsubunit Expressed by a Recombinant Baculovirus
The catalytic subunit of human DNA polymerase (pol) δ, p125, was expressed in recombinant baculovirus-infected insect cells, separated from a baculovirus-encoded DNA polymerase, and was purified to homogeneity by affinity trapping with a histidine-octapeptide at the C-terminus of p125 as the ligand. Purified p125 showed DNA polymerase activity resembling conventionally purified calf thymus pol ...
متن کاملReplication of baculovirus DNA.
The Baculoviridae is a diverse family of pathogens that are infectious for arthropods and are characterized by a complex replication cycle that culminates in the occlusion of virions in a crystalline protein matrix (Blissard & Rohrmann, 1990). Over 400 lepidopteran species serve as hosts for these viruses, with a single virus isolate usually restricted to one or a few related species. In additi...
متن کاملEnzymatic activities of overexpressed herpes simplex virus DNA polymerase purified from recombinant baculovirus-infected insect cells.
Biochemical characterization of the herpes simplex virus (HSV) DNA polymerase, a model DNA polymerase and an important target for antiviral drugs, has been limited by a lack of pure enzyme in sufficient quantity. To overcome this limitation, the HSV DNA polymerase gene was introduced into the baculovirus, Autographa californica nuclear polyhedrosis virus, under the control of the polyhedrin pro...
متن کاملSPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1981
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.40.1.305-308.1981